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10.1111/j.1651-2227.1981.tb05541.x Acta Paediatr Scand

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Reagents and materials Sodium deoxycholate was purchased from Beijing Aoboxing Biotechnology Co., Ltd

For measurement of metabolite levels in cells, hepatocytes were harvested by treating with 0.25 % trypsin (Gibco #25200), washing with ice-cold PBS to remove trypsin, suspending released hepatocytes in ice-cold PBS, and centrifuging the cell suspension at 1600 g for 10 min at 4 C to obtain the pelleted cells, which were immediately frozen and stored at 80 C for later analysis

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