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URL Shaefer CF Jr, Kushner P, Aguilar R

To construct the sorting vector pLKO.1-GST DAF for shRNA gene knockdown experiment, we amplified the coding sequence of N-signal-GST-EGFP-GPI DAF from the pEGFP-GST-GPI DAF plasmid and joined it with FastDigest KpnI/FastDigest BamHI (Thermo Fisher) linearized pLKO.1 vector using ClonExpress II One Step Cloning Kit

Attempts have been made to develop a biotechnological method of folate production for a future switch from current chemical manufacturing to a sustainable fermentative one

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Cancer Cell 5 , 221230 (2004)