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Cysteine is made in the body from serine and methionine and only present in the l-stereoisomer in mammalian proteins

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Methylfolate is required in the activation of tetrahydrobiopterin (i.e., BH4), which is a necessary cofactor for both tryptophan hydroxylase and tyrosine hydroxylase, enzymes required in the synthesis of serotonin and norepinephrine/dopamine, respectively (Figure 1)

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TPO is a heme-dependent protein, thus it contains iron atoms in its structure