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MOTS-c vs SS-31 (Elamipretide) Both target mitochondria

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The dried bacterial pellet was solubilized in 20 ml of B-PER (78243, Thermo Fisher Scientific), supplemented with protease inhibitors (1 g ml 1 of leupeptin, 1 g ml 1 of AEBSF HCl and 1 l of benzonase) and 5 mM imidazole and then transferred to a 50-ml centrifuge tube