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If this happens, try taking them with a small, light snack or dividing your dose throughout the day

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The column was washed with acetonitrile for 10 min followed by a linear gradient back to the initial mixture of 30% acetonitrile with the flow rate 1 ml/min

Seiwerth S, Sikiric P, Grabarevic Z, Zoricic I, Hanzevacki M, Ljubanovic D, Coric V, Konjevoda P, Petek M, Rucman R, Turkovic B, Perovic D, Mikus D, Jandrijevic S, Medvidovic M, Tadic T, Romac B, Kos J, Peric J, Kolega Z