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The percentage activity to the unheated sample was plotted against temperatures for curve fitting
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Oxidized roGFP2 WT , PfGrx C88S , and PfGrx C32S/C88S were produced by incubation of the reduced enzymes with 5 mM Diamide for 60 min on ice
Quantitative Real-Time PCR (qRT-PCR) was used to measure mRNA expression of SASP factors (IL-1, IL-6, TNF-, TGF-) in TM3 cells, normalizing data to GAPDH
It's a tripeptide comprised of three amino acids, i.e
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