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Kabbarah O, Nogueira C, Feng B, Nazarian RM, Bosenberg M, Wu M, et al

D., Schulte, A

[DOI] [PubMed] [Google Scholar] 11.Salbitani G., Vona V., Bottone C., Petriccione M

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After removing the final wash, the beads were incubated twice at room temperature in 80 l of the digestion buffer (2 M urea, 50 mM Tris-HCl, 1 mM DTT and 0.4 g of trypsin) while shaking at 1,000 rpm

Both crystallography studies and structural models suggest that IgG binds to FcRn in an upside-down orientation, such that the Fab arms face down towards the membrane in which FcRn is embedded [34, 61]