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The mutation of GLP-1 positions 16, 17, 18, and 20 into Ala showed minor effects (IC 50 from 0.27 nM to 1.7, 0.46, 0.68, and 1.7 nM

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When investigating the effects of pasireotide and pertussis toxin (PTX), the cells were preincubated with pasireotide for 30 min and PTX for 4 h, respectively 10,11