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The dose-response relationship with sleep disruption appears roughly linear: higher doses correlate with more pronounced effects

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Proper Reconstitution Protocol: Step-by-Step Research Guide For researchers new to peptide reconstitution, following a consistent, sterile protocol is essential

doi:10.1038/210540a0

All mice received 2 days of 10 mL/kg body weight sterile saline subcutaneous injections before beginning the treatment phase to allow for acclimation to the handling and stress associated with injections