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Washing was repeated once before cells were gently resuspended and rested overnight in 1 mL of pre-warmed RPMI 1640 media (Gibco) supplemented with 10% FBS (Biological Industries), L-glutamine (2 mM), penicillin (100 U/mL, Biological Industries) and streptomycin (0.1 mg/mL, Biological Industries), in an incubator at 37 C and 5% CO 2
J., Huang, H
Thinkstock Recilia Vera is vice president of sales at Snowboard Company, a manufacturer of one model of snowboard
Due to the sensitive nature of laboratory reagents, UPLYFT does not accept returns on opened or reconstituted vials
Written informed consent from the patients/participants or patients/participants' legal guardian/next of kin was not required to participate in this study in accordance with the national legislation and the institutional requirements