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Statistically, up to 70% of MVD-affected dogs with mild enlargement will likely never go into heart failure, while dogs with severe enlargement will almost certainly go into heart failure

Then, the cell viability was determined, and 100 L supernatant from cell culture medium was collected to measure LDH content using the specific kit (Nanjing Jiancheng Bioengineering Institute, China) according to the manufacturers protocol [28]

These improvements provide researchers with enhanced tools for studying senescent cell elimination and enable investigation of dose-response relationships and selectivity mechanisms in various experimental models

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It signals fibroblasts to produce collagen and elastin, accelerates wound and tissue remodeling, and shifts gene expression toward repair