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The supernatant was passed through a Ni-Sepharose (Qiagen) packed open-column which was equilibrated with 50 mM Tris, pH8.0, 300 mM NaCl, 1 mM TCEP, 5 mM imidazole

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Republicans are more likely than Democrats to say theyve heard at least some about the administrations recent deals with pharmaceutical companies to lower the cost of some prescription drugs for Medicaid (44% v

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