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Flow cytometry For cell cycle analysis, 5 10 5 cells were harvested by trypsinization (Beyotime, China), washed twice with PBS, and then fixed with 75% ice-cold ethanol for 24 hours at 4 C

Do not freeze at any stage, do not expose to ambient for more than brief periods, and discard any solution that develops particulates or discoloration

O., Giri, N., McReynolds, L

Asian Journal of Andrology 1-8

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Injecting growth hormone directly has been linked to type 2 diabetes, gynecomastia (man boobs), swelling and joint pain, and a higher risk of certain cancers (4)