The qRT-PCR reaction was made up of 4.5 L diluted (20X) cDNA template, 0.25 L forward and 0.25 L reverse primers of GSTs, and 5 L of Maxima SYBR Green qPCR Master Mix (2X) (Thermo Scientific, Waltham, MA, USA) in double-distilled water at a final volume of 10 L
What are the next steps
Rizo JA, Spencer TE, Kelleher AM (2024) Protocol for the establishment and characterization of an endometrial-derived epithelial organoid and stromal cell co-culture system
Gelatin forms a familiar gel like texture (jello-o!) when mixed with water, while collagen does not
When supplemented with an inactive form, it is converted to the active form in the body
These growth factors promote endothelial cell migration and new blood vessel formation, addressing the critical vascular requirement for bone healing