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To inhibit de novo protein synthesis, cycloheximide (50 g/ml, Sigma-Aldrich cat no O1810) was added to the cells, and they were then incubated at 4 o C for 10 min

While it has potential advantages, not everyone may find it the best choice

Rzehak P et al

No NAFLD patient had clinical evidence of hepatic decompensation, such as hepatic encephalopathy, ascites, variceal bleeding, or a serum bilirubin level greater than twice the upper limit of normal

This elevated baseline body weight and helped buffer against anticipated losses and pharmacological effect, allowing the research animals to complete the study without requiring dose holidays or protocol adjustments

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