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doi: 10.1210/en.2005-0102 100 MussoGGambinoRDurazzoMBiroliGCarelloMFagEet al

The procedure was performed five times for each sample

The eluted hFSP1 proteins were treated with Ulp1 to cleave off the 6his-SUMO tag, dialyzed against buffer A, re-loaded onto the Ni-Chelating Sepharose Fast Flow resin, and then the flow-through was concentrated and further purified by Superdex 200 Increase 10/300 GL columns (GE Healthcare, USA) with buffer B

10.1016/j.bios.2018.05.040 Biosens

Snchez et al., 2017)

[link] Nishida T, Kubota S, Aoyama E, Yamanaka N, Lyons KM, Takigawa M., "Low-intensity pulsed ultrasound (LIPUS) treatment of cultured chondrocytes stimulates production of CCN family protein 2 (CCN2), a protein involved in the regeneration of articular cartilage: Mechanism underlying this stimulation", Osteoarthritis Cart 25: 759-769 (2017)