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It has been proposed that this serves to reroute metabolic intermediates into the pentose phosphate pathway to facilitate NADPH generation and adaptation to oxidative stress [38]

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Finally, the 3-Cy3 labelling reaction was performed overnight at 4C in 20 l containing 100 pmol unlabeled RNA, 0.5 mM ATP, 200 pmol pCp-Cy3 (Jena Bioscience), 10 U RiboLock RNase Inhibitor (Thermo Fisher Scientific), 1x Ligation Buffer and 10 U T4 RNA Ligase (Thermo Fisher Scientific)

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Others might choose to use machines, because their shoulders are not too healthy and so need the extra stability a machine can provide