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A further observation of this study relates to the capacity of the receptor serine doublet phospho-sites to regulate its dynamic behaviour under non-stimulated conditions, as observed in our RICS analysis of receptor plasma membrane diffusion, which demonstrated significantly reduced basal diffusion rate of the triple AA phospho-null mutant compared to the WT receptor under vehicle conditions

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Elsouri KN, Heiser SE, Cabrera D, Alqurneh S, Hawat J, Demory ML, Demory-Beckler M

Understanding these factors helps researchers correctly interpret divergent pharmacokinetic observations and adjust experimental designs accordingly