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Sections were blocked with Tris buffered saline (TBS) containing 1% BSA(Yeasen, Shanghai, China) and 10% goat serum (Sangon Biotech, Shanghai, China) for 60 min

Dry mass (DW) of the leaf discs was determined by drying the tissues at 70 C for 2 to 4 d

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Blood was collected in EDTA-coated microvette tubes, chilled on ice and centrifuged at 3,000 g and 4 C for 10 min

doi: 10.1016/j.coph.2013.09.006 182 BarreraJGJonesKRHermanJPDAlessioDAWoodsSCSeeleyRJ

GLP-1 may increase energy expenditure by activating brown adipose tissue (BAT) thermogenesis through sympathetic nervous system activity induced by GLP-1 activity in the central nervous system (CNS) [108]