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Tube formation assay To investigate tube formation, 250 L of thawed Matrigel (abs9493, Absin) was added to each lower chamber of a 24-well plate
Health conditions, such as diabetes
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Owens et al., 2011)
Primary antibodies included anti--Catenin, anti-GSK3, anti-phospho-GSK3, anti-GAPDH, and anti--tubulin (Boster Biological Technology, Pleasanton, CA, United States), anti-Bcl2, anti-Bax, and anti-Cyclin D1 (Abcam plc, Cambridge, United Kingdom), anti-Akt and anti-phospho-Akt (Cell Signaling Technology, Danvers, MA, United Ststes), anti-Wnt10b, anti-CDK2, anti-CDK4, and anti-P21 (ABclonal, Wuhan, China), and anti-ki67 (Proteintech, Wuhan, China)
