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Every batch of LiveWell IGF-1 LR3 is third-party tested and ships with a Certificate of Analysis (COA), typically 99%+ purity by HPLC

Prior to each run the column was equilibrated with SEC buffer (25 mM Tris-HCl pH 7.5, 150 mM NaCl, 2 mM -mercaptoethanol)

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Consent for publication Not applicable in this review

What that looks like in practice: A face-to-face consultation first, never a same-day injection

Critically, when we introduced mutations into the Fc that reduce FcRIIIa bindingwhile retaining the cysteine at position 330and co-expressed this construct with the cysteine-mutated FcRIIIa soluble domain, we failed to produce the complex despite robust expression of both components, indicating that the native interaction is essential for guiding and stabilizing the intermolecular disulfide (Supplementary Fig