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Pull-down assay by FLAG-Sirt7 To a 150 L of solution of nucleosomes (0.05 M) and FLAG-Sirt7 (WT, DM, or CA) (0.17 ng/L) in buffer A (20 mM Tris (pH 8.0), 100 mM NaCl, 5 mM MgCl 2 , 1 mM DTT, 2.5% glycerol, 0.01% Triton X-100), 5 L of anti-FLAG M2 magnetic beads (Sigma, #M8823) in 50 L of buffer A was added

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Also, the fact that many of those antioxidants cannot be synthesized within human cells due to lack of enzymes in the first place, qualify them as essential nutrients for our population

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