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In step-c), Fmoc-Thr(tBu)-Phe-Thr(tBu)-Ser(tBu)-Asp(OtBu)-VaI-Ser(Oxa)-OH (fragment-2) was condensed with peptide obtained from step-b) in presence of a coupling agent to obtain Thr(tBu)-Phe-Thr(tBu)-Ser(tBu)-Asp(OtBu)-VaI-Ser(Oxa)-Ser(tBu)-Tyr(tBu)-Leu-Glu(OtBu)- Gly-Gln(Trt)-Ala-Ala-Lys(C18 diacid mono-t-butyI-y-Glu(AEEA-AEEA)-OtBu)-Glu(OtBu)- Phe-Ile-Ala-Trp (Boc)-Leu-Val-Arg (pbf) Gly-Arg (pbf)-Gly-OtBu peptide

Maccaferri G, Dingledine R (2002)

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