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Plasma glucagon levels were quantified using the Mercodia Glucagon ELISA kit (10-1281-01, Mercodia)

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Longstaff (European Chemical Industry Ecology and Toxicology Centre), ICI Central Toxicology Laboratory, Genetic Toxicology Section, Macclesfield, United Kingdom Dr M

[PMC free article: PMC8281430] [PubMed: 34307603] (Systematic review of the literature on herb induced liver injury identified 446 references describing 936 cases due to 79 different herbal products, the most common being He Shou Wu [91], green tea [90] Herbalife products [64], kava kava [62] and greater celandine [48]

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Recommended Aseptic Practices Single-use needles : Never reuse needlesrepeated punctures damage rubber stoppers, allowing microbial ingress and losing vacuum seal Alcohol swabbing : Swab stopper before each puncture and allow alcohol to fully evaporate (30 seconds) before inserting needle Syringe selection : Use luer-lock syringes rather than slip-tip to prevent accidental needle detachment during withdrawal Withdrawal technique : Insert needle at a slight angle to minimize coring of rubber stopper particles into solution Air displacement : Inject an equal volume of air into the vial before withdrawing liquid to maintain neutral pressurethis prevents stopper collapse in multi-dose vials Signs of Microbial Contamination Even with bacteriostatic water, gross contamination can occur with poor technique: Cloudiness that develops over 24-48 hours (peptide precipitation is immediate