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The eluted protein was digested overnight with thrombin, followed by anion-exchange chromatography (HiTrap Q FF, Cytiva), and finally polished by SEC using a Superdex 200 column (HiLoad 16/600, Cytiva)

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Identifying an essential role of nuclear LC3 for autophagy

requires daily dosing, has significant alcohol interaction, and is less effective for some patients PT-141 (bremelanotide): MC3R/MC4R agonist acting on hypothalamic sexual motivation circuits