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We identified AEP trails as having distinct morphology (distinguishable from membrane vesicles and tubule-like outer membrane protrusions, as well as the S-motility-related EPS fibrils) in the TEM, and by their pH sensitivity, as treatment with acidic stains (un-buffered uranyl acetate (UA), pH 4.5 or SiPTA at pH 4.0) removed slime trails (but not other membrane components, i.e., vesicles) from grids, while neutral stains (SiPTA at pH 7.0) did not (Fig
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These immune alterations parallel mechanisms in rheumatoid arthritis, where pro-inflammatory cytokines (TNF-, IL-6) and immune cell dysfunction drive chronic inflammation (28)
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