Methods Animal housing Male and female wild-type C57BL/6J mice were purchased (The Jackson Laboratory, Bar Harbor, USA) and bred in-house
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Anti-inflammatory and Immunomodulatory Effects (IL-1, IL-6, TNF-) Inflammation is a natural and necessary part of the healing process, but chronic or excessive inflammation can impede tissue repair and contribute to pain and tissue degradation
Sample preparation (B) In an alternate protocol, the frozen liver tissue was directly lysed by probe sonication (2 10 pulse, 40% power output) in 4 M urea/DPBS and briefly cleared of debris by centrifugation (5000 g , 5 min, 4 C)
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