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Ammonia values do not correlate with severity and are easily influenced by the measuring method utilized

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Cytoplasmic free Ca 2+ concentration imaging For live-cell cytoplasmic free Ca 2+ concentration ([Ca 2+ ] i ) imaging experiments, Gcg-GCaMP6f islets were immobilised to a poly-l-lysine-coated coverslip fixed in a custom-built imaging chamber filled with EC3 (ESM Table 2)

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