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( 1 to construct a calibration curve for determining flavonoid content, with average R 2 = 0.9916 ( Figure 1B ) ( Figure 1 2.2.2 Gas chromatography-mass spectrometry sample derivatization and analysis Before application to gas chromatography-mass spectrometry (GC-MS), the grape seed extract (GSE) sample was extracted, prepared, and treated with a silylation reagent to derivatize the functional groups into trimethylsilyl (TMS) derivatives ( 2.2.3 LC/MS/MS conditions and parameters (instrument, ionization mode) The GSE was dissolved and analyzed using liquid chromatographyelectrospray ionizationtandem mass spectrometry (LC-ESI-MS/MS, SCIEX Triple Quad 5500+ MS/MS)

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