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If you accidentally freeze a vial, discard it

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For instance, in the presence of the antibiotic metronidazole, thiaminase I substitutes the thiazolium ring of thiamine with the imidazolium ring of metronidazole, resulting in a thiamine antagonist which can inhibit TPK (160)

The urea was diluted to a final concentration of 2 M by adding 50 mM ammonium bicarbonate (NH 4 HCO 3 ) (Sigma-Aldrich, Saint-Louis)

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