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Specifically, bloodletting puncture can suppress the expression of MAPK, ERK, p38, and their downstream molecules, MMP9 and AQP4, leading to enhanced neurological function, reduced brain edema, stabilization of the bloodbrain barrier, and decreased apoptosis

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This comprehensive guide has walked through the critical steps, from understanding the lyophilized form to executing a precise reconstitution, and finally, to implementing best practices for storage and handling