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These include insulin resistance, impaired appetite signaling, and chronic low-grade inflammation, all of which can make weight loss more difficult

For apoptosis assay, cells were suspended in Annexin V binding buffer (BD Science, New Jersey, USA) containing Annexin V-FITC (MBL International, Woburn, MA, USA) and propidium iodide (BD Science) for 15 min in dark

Biocon is also developing a rival to Eli Lilly s obesity treatment Mounjaro/Zepbound , containing the active ingredient tirzepatide, though timing and marketing plans were not provided

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