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While medications are initially created and clinically tested to achieve Food and Drug Administration (FDA) approval for a specific medical condition, physicians often find that a new drug is useful in additional unexpected ways

In vitro characterization is classically done on cloned human receptors expressed in cell lines (HEK293, CHO) via cAMP measurements (ELISA, FRET) or beta-arrestin recruitment (BRET, NanoBiT)