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The 3F52 VL and VH regions were PCR amplified and cloned by standard restriction-based cloning into a toolbox vectors carrying a human kappa light chain CL or a truncated human IgG4 CH, respectively

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The best bac water for peptides is medical-grade bacteriostatic water sterile water preserved with 0.9% benzyl alcohol to prevent bacterial contamination during multi-use reconstitution

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