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RNA isolation and RT-qPCR Total RNA was extracted from pooled retinal tissue from 2 eyes of a single adult fish (individual fish served as a biological replicate) using the RNeasy Plus Mini Kit (Qiagen, Germantown, MD) according to the manufacturers protocol for animal tissue

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Dosing Notes Empty stomach works best or at least 30 minutes before eating

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Red Blood Cell Formation: Much of the bodys vitamin B12 content is sequestered to the bone marrow for the all-important task of creating new red blood cells

However, instead of pinching your fat, hold the flesh firmly between your thumb and index finger and insert the needle in the center