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The other transgenic fish lines, including Tg(mlc2:LMNA(WT);myl7:EGFP) , Tg(mlc2:LMNA(L35P);myl7:EGFP) , Tg(mlc2:LMNA(E358K);myl7:EGFP) , Tg(mlc2:LMNA(R453W);myl7:EGFP) , Tg(mlc2:LMNA(W520G);myl7:EGFP) , and Tg(mlc2:LMNA(A539V);myl7:EGFP) , were generated by using the Tol2 Gateway cloning Toolkit as described previously 30,31

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