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That logic is technically correct

Samples were incubated overnight at 4C, rinsed 5 in 1xPBS + 0.1% Triton X-100, then incubated overnight at 4C in secondary antibody + 10% NGS in 1xPBS + 0.1% Triton X-100

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People who are fatigued or stressed become intoxicated more quickly than people who are rested and relaxed

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Abdel Fattah NSA, Ebrahim AA, El Okda ES