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In MCF-7 cells, the largest synergy was observed between EA and PRT 4165

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The reaction mixture was retrotranscribed at 48 C for 45, denatured for 2 at 94 C and then subjected to 1826 cycles of denaturation for 30 seconds, annealing at 55 C for 45 seconds, and elongation at 68C for 1 minute