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Then, membranes were incubated with appropriate secondary antibodies, HRP-goat-anti-mouse (Abcam, ab6789) or HRP-goat-anti-rabbit (Abcam, ab6721), to bind the primary antibodies for 1 h at room temperature
One such enzyme is cystathionine beta-synthase (CBS), which catalyzes the first and most important (rate-limiting) step in trans-sulfuration from homocysteine to cystathionine
However, the disruption of redox balance may result from increased ROS generation and/or decreased levels of antioxidants thus elevated ROS level results in oxidation of macromolecules, such as proteins, lipids, and nucleic acids, which subsequently leads to DNA damage, disturbed signal transduction, cytotoxicity, and cell death (Ray et al., 2012
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