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In relation to the bone, IGF-1 is an important mediator of the effects of GH and modulates bone growth through its paracrine action in the growth plate (220) as it was demonstrated that the selective deletion of the igf1 gene in the liver and a consequent decrease in systemic IGF-1 did not affect the animals body and femoral growth (221)

K1 cells were first treated with RSL3 for 6 h

A unit of 40 g of protein lysate was incubated with SDS sample buffer for 5 min at 90 C, resolved on a 10% polyacrylamide gel and electroblotted on a polyvinylidene difluoride membrane

10.1016/j.euroneuro.2004.09.001 66

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