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It is envisioned that a specific AIE probe can be designed by decorating the TPE core with a particular functional group 26,57

The amount of enzyme and incubation time must be empirically determined for each fusion protein

The PZQ LD 50 for NTS was determined after just 24 h exposure using both the Cell TiterGlo (27.1 2.27 M, Table 2) and the phenotypic analysis (42.9 0.83 M, Supplementary Movie 2), which is also used by others 54

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Furthermore, suboptimal reaction kinetics under standard conditions have been documented for certain antioxidants (Celik et al., 2010)

The remaining variable, activity factor, serves as a multiple for the individuals resting metabolic rate that is calculated by the formula