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We observe that alanine mutants of CCR8 residues involved in CCL1 binding display varying degrees of functional impairment in a CCL1-induced cAMP inhibition assay, with mutations Y172A 4.64 , D178A ECL2 , Q182A ECL2 , Y184A ECL2 , and H283A 7.36 having the strongest effect within the mutants we tested

doi: 10.1016/j.cell.2017.02.004