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Supernatants were incubated overnight with anti-GFP agarose beads (Chromotek, GTA-100), beads were then washed several times, and incubated for 10 min at room temperature with 1 M hydroxylamine, pH 7.4 (Sigma-Aldrich, 159417) or 2 M Tris-HCl, pH 7.4

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The three sites are located in Indiana, Brussels and Anagni, Italy

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Other TNF blockers can also weaken it