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These proteins are initially synthesized as precursors that associate with the endoplasmic reticulum and nuclear membranes, with SREBP-1c representing the most abundant isoform in hepatic tissue (Ceni et al., 2014)
In many experimental animal models, MTX has been found to increase serum urea and creatinine levels (Reference Moodi, Hosseini and Abedini16,Reference Tousson, Zaki and Abu-Shaeir50,Reference Jafaripour, Naserzadeh and Alizamani51) , but there are also studies showing that MTX reduces serum urea concentration (Reference Budancamanak, Kanter and Demirel52Reference Severin, Campagno and Brandoni54)
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