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C., Gonzlez-Sarras, A., and Toms-Barbern, F

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Enhancing endogenous NO production should provide physiological and more sustained effects

Stereological brain sampling of the areas of interest was performed and brain were serially cut on a cryostat (Leica Biosystems, Germany, cat# CM3050S), mounted on Superfrost Plus slides (Thermo Fisher Scientific, Reinach, Switzerland), and cryoprotected in 50% of PB, 30% of ethylene glycol, 20% of glycerol, and subsequently stored at 20 C

500-900 per tube

If accurate, that points to a signaling or regulatory effect rather than a direct hypnotic one