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coli containing plasmid pANT35 or pANT62 was inoculated from an overnight culture at a ratio of 1:100 in a 2 l baffled Erlenmeyer flask filled with 400 ml Zym-5052 medium 51 containing 50 g ml 1 kanamycin sulfate per flask, incubated at 20 C, 140 rpm, 5 cm shaking diameter for 24 h, and the final OD 600 was determined using a CO8000 cell density meter (WPA biowave)

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Bleeding in the upper GI tract lasts longer, so the blood may be dark, black and tarry

This suggests that the peptide may have therapeutic potential for conditions like osteoarthritis, cardiovascular disease, and neurodegenerative disorders

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Ileus Ileus is a serious intestinal condition where the bowels stop moving properly, leading to painful blockages