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The lower limit of detection for this assay was 0.016 M or 0.13 nmol/mg protein

Baseline photographs from 4+ angles with consistent lighting Progress photos at 2-4 week intervals Daily shedding observations during washing Scalp feel assessments noting any changes in texture or oiliness Hair texture notes including thickness, strength, and shine Side effect logging including injection site reactions Overall subjective satisfaction ratings Hair density counts provide more objective measurements for those wanting detailed tracking

Background HO level was 143,596 RLU

Why micrograms matter here: retatrutide doses are typically discussed in milligrams (1mg, 2mg, 4mg, 8mg, 12mg) at the protocol level, but when you are physically drawing from a syringe, you are working in fractions of a milliliter