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Shaking creates foam, introduces air bubbles, and causes physical stress that degrades the peptide through a process called denaturation

Then Tween 20 was added to the mixture gradually, the mixture was homogenized to form nanoemulsions by a high speed homogenizer (Model: 400ELPC, PRO Scientific Inc., 01-02411ELPC homogenizer, USA) at 20,000 rpm for 8 min

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Additionally, it is essential to store peptides in tightly sealed vials or containers to minimize exposure to air and moisture, which can lower their quality

But again, it takes them 12 to 18 months to fix it