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After a 20-min incubation with immunol staining blocking buffer (Beyotime, Shanghai, China), cells were continuously incubated with mouse anti-SMA (1:100, Abcam) and rabbit anti-CD133 (1:100, Abnova) 44 at 4 C overnight

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Results Using 53 search terms related to digital health and the ten selected rare diseases (see Supplementary Table 1), a total of 8373 clinical trial records and publications were initially retrieved from Clinicaltrials.gov, the ICTRP, Cochrane, PubMed and Web of Science

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