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Sections were washed 6 10 min with KPBS and placed in goat anti-rabbit AF Plus 488 secondary antibody (1:500, Invitrogen) for 2 h at room temperature

Studies have highlighted that the conversion of glucose to lactate by cells constitutes a tightly regulated metabolic state, which may confer advantages during periods of heightened biosynthetic demand (29,30)

Mistake 2: Shaking the vial to dissolve the powder faster Shaking introduces air bubbles and creates shearing forces that can break apart the peptide structure

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Everyones pain tolerance is different

Fatiqin A, Amrulloh H, Simanjuntak W